immunology UPDATE
Sequence determinants of innate immune activation by short interfering RNAs
Background:
Short interfering RNAs (siRNAs) have been shown to induce immune stimulation through a number of different receptors in a range of cell types. In primary cells, both TLR7 and TLR8 have been shown to recognise siRNAs however the precise sequence requirements for their activation have not yet been elucidated.
Results:
A total of 207 siRNA sequences [...]
Enrichment and analysis of secretory lysosomes from lymphocyte populations
Background:
In specialized cells, such as mast cells, macrophages, T lymphocytes and Natural Killer cells in the immune system and for instance melanocytes in the skin, secretory lysosomes (SL) have evolved as bifunctional organelles that combine degradative and secretory properties. Mutations in lysosomal storage, transport or sorting molecules are associated with severe immunodeficiencies, autoimmunity and (partial) [...]
Conserved cryptic recombination signals in Vkappa gene segments are cleaved in small pre-B cells
Background:
The cleavage of recombination signals (RS) at the boundaries of immunoglobulin V, D, and J gene segments initiates the somatic generation of the antigen receptor genes expressed by B lymphocytes. RS contain a conserved heptamer and nonamer motif separated by non-conserved spacers of 12 or 23 nucleotides. Under physiologic conditions, V(D)J recombination follows the "12/23 [...]
The orphan adapter protein SLY1 as a novel anti-apoptotic protein required for thymocyte development
Background:
SH3 containing Lymphocyte Protein (SLY1) is a putative adapter protein exclusively expressed in lymphocytes which is involved in antigen receptor induced activation. We previously have generated SLY1?/? mice harbouring a partial deletion in the N-terminal region of SLY1 which revealed profound immunological defects in T and B cell functions.
Results:
In this study, T cell development in [...]